IJIMS.2016.108

Type of Article: Original Research

Volume 3; Issue 3: March 2016

Page No.: 242-247

DOI: 10.16965/ijims.2016.108

 

Antioxidant Effect of Nigella Sativa Seed Powder and Thymoquinone in Normal and Sterptozotocine Induced Diabetic Albino Rats

Shaik Hussain Saheb *1, Desai SD 2, Kusal K Das 3, Haseena S 4.

*1 Ph.D Scholar, Department of Anatomy, Shri B M Patil Medical College Hospital & Research Centre, Bijapur and Assistant Professor in Anatomy, JJM Medical College, Davangere,  Karnataka, India.

2 Principal & Professor of Anatomy, Shridevi Institute of Medical Sciences and Research Hospital, Tumkur,  Karnataka, India.

3 Professor, Department of  Physiology,  Shri B M Patil Medical College Hospital & Research Centre, Bijapur, Karnataka, India.

4 Ph.D Scholar, Department of Physiology, Shri B M Patil Medical College Hospital & Research Centre, Bijapur and Lecturer in Physiology, JJM Medical College, Davangere,  Karnataka, India.

CORRESPONDING AUTHOR ADDRESS: Mr. Shaik Hussain Saheb, Assistant Professor in Anatomy, JJM Medical College, Davangere,  Karnataka, India. Mobile no.: +91-9242056660 E-Mail: anatomyshs@gmail.com

ABSTRACT

Introduction: Malondialdehyde (MDA) cause toxic stress in cells and form covalent protein and its used as a biomarker to measure the level of oxidative stress. There is opposite mechanism which control the flux of reactive oxygen species (ROS) called as antioxidant system, which includes both enzymatic andnon-enzymatic components. The important enzyme of this system is superoxide dismutase (SOD).  The black cumin or Nigella Sativaseeds are used in traditional medicine of different countries. Thymoquinone is major bioactive component of  nigellasativa seed and it is the cause of therapeutic property of Nigella sativa seed. The present study is conducted to see the effect of Nigella Sativa seed powder and Thymoquinone  on Serum MDA and SOD levels in normal and Streptozotocine Induced Diabetic Rats.

Materials and Methods: 36 rats were selected for this study and divided in to 6 groups each contains 6 rats, one group served as normal control, one group served as normal rats received the nigella sativa seed powder(300mg/Kg BW), one groups served as normal rats received the Thymoquinone(4mg/kg BW), one group served as Streptozotocine(50mg/kg BW) induced diabetic control rats,  one group served as diabetic rats  received the nigella sativa seed powder(300mg/Kg BW) and one groups served as diabetic rats received the Thymoquinone(4mg/kg BW).

Results: There is no change in MDA and SOD levels in normal rats treated with nigella sativa seed powder and thymoqinone. The levels of MDA are increased significantly in diabetic rats compared with normal rats, when the diabetic groups treated with nigella sativa seed powder and thymoqinone the MDA levels are decreased significantly(p<0.05). The levels of SOD are decreased significantly in diabetic rats compared with normal rats, when the diabetic groups treated with nigella sativa seed powder and thymoqinone the SOD levels are increased significantly.

Conclusion: The results of these study concluding that nigella sativa seed powder and thymoquinone has having antioxidant effect in diabetic rats which lowering the MDA levels and increasing the SOD levels in streptozotocine induced diabetic rats.

Key Words: MDA, SOD, Nigella Sativa seed, Antioxidant. Thymoquinone.

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