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ISSN: 2394-4137 (E) 2394-6318 (P)

IJIMS.2016.108

Type of Article: Original Research

Volume 3; Issue 3: March 2016

Page No.: 242-247

DOI: 10.16965/ijims.2016.108

 

Antioxidant Effect of Nigella Sativa Seed Powder and Thymoquinone in Normal and Sterptozotocine Induced Diabetic Albino Rats

Shaik Hussain Saheb *1, Desai SD 2, Kusal K Das 3, Haseena S 4.

*1 Ph.D Scholar, Department of Anatomy, Shri B M Patil Medical College Hospital & Research Centre, Bijapur and Assistant Professor in Anatomy, JJM Medical College, Davangere,  Karnataka, India.

2 Principal & Professor of Anatomy, Shridevi Institute of Medical Sciences and Research Hospital, Tumkur,  Karnataka, India.

3 Professor, Department of  Physiology,  Shri B M Patil Medical College Hospital & Research Centre, Bijapur, Karnataka, India.

4 Ph.D Scholar, Department of Physiology, Shri B M Patil Medical College Hospital & Research Centre, Bijapur and Lecturer in Physiology, JJM Medical College, Davangere,  Karnataka, India.

CORRESPONDING AUTHOR ADDRESS: Mr. Shaik Hussain Saheb, Assistant Professor in Anatomy, JJM Medical College, Davangere,  Karnataka, India. Mobile no.: +91-9242056660 E-Mail: anatomyshs@gmail.com

ABSTRACT

Introduction: Malondialdehyde (MDA) cause toxic stress in cells and form covalent protein and its used as a biomarker to measure the level of oxidative stress. There is opposite mechanism which control the flux of reactive oxygen species (ROS) called as antioxidant system, which includes both enzymatic andnon-enzymatic components. The important enzyme of this system is superoxide dismutase (SOD).  The black cumin or Nigella Sativaseeds are used in traditional medicine of different countries. Thymoquinone is major bioactive component of  nigellasativa seed and it is the cause of therapeutic property of Nigella sativa seed. The present study is conducted to see the effect of Nigella Sativa seed powder and Thymoquinone  on Serum MDA and SOD levels in normal and Streptozotocine Induced Diabetic Rats.

Materials and Methods: 36 rats were selected for this study and divided in to 6 groups each contains 6 rats, one group served as normal control, one group served as normal rats received the nigella sativa seed powder(300mg/Kg BW), one groups served as normal rats received the Thymoquinone(4mg/kg BW), one group served as Streptozotocine(50mg/kg BW) induced diabetic control rats,  one group served as diabetic rats  received the nigella sativa seed powder(300mg/Kg BW) and one groups served as diabetic rats received the Thymoquinone(4mg/kg BW).

Results: There is no change in MDA and SOD levels in normal rats treated with nigella sativa seed powder and thymoqinone. The levels of MDA are increased significantly in diabetic rats compared with normal rats, when the diabetic groups treated with nigella sativa seed powder and thymoqinone the MDA levels are decreased significantly(p<0.05). The levels of SOD are decreased significantly in diabetic rats compared with normal rats, when the diabetic groups treated with nigella sativa seed powder and thymoqinone the SOD levels are increased significantly.

Conclusion: The results of these study concluding that nigella sativa seed powder and thymoquinone has having antioxidant effect in diabetic rats which lowering the MDA levels and increasing the SOD levels in streptozotocine induced diabetic rats.

Key Words: MDA, SOD, Nigella Sativa seed, Antioxidant. Thymoquinone.

REFERENCES

  1. Padhye S, Banerjee S, Ahmad A, Mohammad R and Sarkar FH. From here to eternity the secret of Pharaohs: Therapeutic potential of black cumin seeds and beyond. Cancer Ther., 2008: 495-510.
  2. Randhawa MA and Alghamdi MS. Anticancer activity of Nigella sativa (Black seed) A Review. J. Clin. Med. 2011; 39(6): 1075-1091.
  3. Al-Ali A, Alkhawajah A, Randhawa MA and Shaikh NA. Oral and intraperitoneal LD50 of thymoquinone, an active principle of Nigella sativa, in mice and rats. Ayub Med. Coll. 2008;20(2): 25-27.
  4. Houghton PJ, Zarka R, De-Las-Heras B, Hoult JR. Fixed oil of Nigella sativa and derived thymoquinone inhibit Eicosanoid generation in leukocytes and membrane lipid peroxidation. Planta Med 1995; 61: 33-36.
  5. Hassan M, El-Dakhakhny M. Effect of some Nigella sativa constituents on chemical carcinogenesis in hamster cheek pouch. J Egypt Soci Pharmacol Exp Ther 1992; 11:675–677.
  6. El-Tahir KEH, Al-Harbi MMS, Ashour MM. The cardiovascular actions of the volatile oil of the black seed in rats: elucidation of the mechanism of action. Gen Pharmacol. 1993;24:1123–1131.
  7. Bray TM, Bettger WJ. The physiological role of zinc as an antioxidant. Free Radic Biol Med. 1990;8:281–291.
  8. Kanter M, Meral I, Dede S, Gunduz H, Cemek M, Ozbek H. Effects of Nigella sativa and Urtica dioica L. on lipid peroxidation, antioxidant enzyme systems and some liver enzymes in CCl4- treated rats. J Vet Med A Physiol Pathol Clin Med. 2003;50:264–268.
  9. Houghton PJ, Zarka R, De-Las-Heras B, Hoult JR. Fixed oil of Nigella sativa and derived thymoquinone inhibit Eicosanoid generation in leukocytes and membrane lipid peroxidation. Planta Med 1995; 61: 33-36.
  10. Hassan M, El-Dakhakhny M. Effect of some Nigella sativa constituents on chemical carcinogenesis in hamster cheek pouch. J Egypt Soci Pharmacol Exp Ther 1992; 11:675–677
  11. Mansour MA, Ginawi OT, El-Hadiyah T, El-Khatib AS, Al-Shabanah OA, Al- Sawaf HA. Effects of volatile oil constituents of Nigella sativa on carbon tetrachloride-induced hepatotoxicity in mice: evidence for antioxidant effects of thymoquinone. Res Commun Mol Pathol Pharmacol 2001; 110: 239-251.
  12. Nagi MN, Alam K, Badary OA, Al-Shabaneh OA, Al-Sawaf HA, Al-Bekairi AM. Thymoquinone protects against carbontetra chloride hepatotoxicity in mice via an antioxidant mechanism. Biochem Mol Biol Int 1999, 47: 153-159.
  13. Mansour MA, Nagi MN, El-Khatib AS, Al-Bekairi AM. Effects of thymoquinone on antioxidant enzyme activities, lipid peroxidation and DT-diaphorase in different tissues of mice: a possible mechanism of action. Cell Biochem Funct 2002; 20: 143-151.
  14. W. Baynes and S. R. Thorpe, “Role of oxidative stress in diabetic complications: a new perspective on an old paradigm,”Diabetes, vol. 48, no. 1, pp. 1–9, 1999.
  15. Dandona, K. Thusu, S. Cook, et al., “Oxidative damage to DNA in diabetes mellitus,”The Lancet, vol. 347, no. 8999, pp. 444–445, 1996.
  16. Sakurai and S. Tsuchiya, “Superoxide production from nonenzymatically glycated protein,”FEBS Letters, vol. 236, no. 2, pp. 406–410, 1988.
  17. Brownlee, “Biochemistry and molecular cell biology of diabetic complications,”Nature, vol. 414, no. 6865, pp. 813–820, 2001.
  18. Harrison, K. K. Griendling, U. Landmesser, B. Hornig, and H. Drexler, “Role of oxidative stress in atherosclerosis,”The American Journal of Cardiology, vol. 91, no. 3, pp. 7A–11A, 2003.
  19. K. M. Mohazzab, P. M. Kaminski, and M. S. Wolin, “NADH oxidoreductase is a major source of superoxide anion in bovine coronary artery endothelium,”American Journal of Physiology, vol. 266, no. 6, pp. H2568–H2572, 1994.
  20. Hayoz, D.; Ziegler, T.; Brunner, H.R. and Ruiz, J. Diabetes mellitus and vascular lesions. Metabolism. 1998;47:16–19.
  21. Bonnefont, D.; Bastard, J.P.; Jaudon, M.C. and Dellattre, J. Consequences of diabetes status on the oxidant ⁄ antioxidant balance. Diabetes Metab. 2000;26: 163-76.
  22. Rains, J.L. and S.K. Jain. Oxidative stress, insulin signaling, and diabetes. Free. Radic. Biol. Med. 2011;50: 567-575.
  23. Mates JM, Perez-Gomez C, NunezDeCastro I. Antioxidant enzymes and human diseases. Clin Biochem 1999; 32:595 –603.
  24. Desai S D, Shaik Hussain Saheb, Kusal K Das, Haseena S. Effect of Thymoquinone on MDA and SOD levels in Sterptozotocine Induced Diabetis Albino Rats. Pharm. Sci. & Res. Vol. 7(4), 2015, 206-209.
  25. Desai S D, Shaik Hussain Saheb, Kusal K Das, Haseena S. Effect of Nigella Sativa Seed Powder on MDA and SOD levels in Sterptozotocine Induced Diabetis Albino Rats. Pharm. Sci. & Res. Vol. 7(4), 2015, 523-526.
  26. Haseena S, Manjunath Aithal, Kusal K Das, Shaik Hussain Saheb. Phytochemical Analysis of Nigella Sativa and it`s effect on reproductive system. J. Pharm. Sci. & Res. Vol. 7(8), 2015, 514-517.
  27. Desai S D, Shaik Hussain Saheb, Kusal K Das, Haseena S. Phytochemical Analysis of Nigella Sativa and it`s Antidiabetic Effect. J. Pharm. Sci. & Res. Vol. 7(8), 2015, 527-532.
  28. Nadiger Ha, Marcus Sr, ChandrakalaMv, Kulkarni Dd. Malondialdehyde Levels In Different Organs Of Rats Subjected To Acute Alcohol Toxicity. Indian Journal of Clinical Biochemistry 1986; 133-136.
  29. Marklund, S., Marklund, G. Involvement of the superoxyde anion radical in the auto oxidation of pyrogallol and a convenient assay for superoxyde dismutase. Eur. J. Biochem. 1974; 47:469-474.
  30. Murugesan, M. Ragunath, T. Prabu, S. Nadanasabapathi, M. Sakthivel, V. Manju. Protective role of black cumin (Nigella sativa) on isoproterenol induced myocardial infarction in rats. International Journal of Pharmacology and Clinical Sciences. 2012;1(2 ):45-53.
  31. Abdelmeguid NE,Fakhoury R, Kamal SM, Al Wafai RJ. Effects of Nigella sativa and thymoquinone on biochemical and subcellular changes in pancreatic β-cells of streptozotocin-induced diabetic rats. J Diabetes. 2010 Dec;2(4):256-66.
  32. Edibe Sariciceka, Mehmet Tarakcioglub, Vahap Saricicekc, Murat Taner Gulsend, Metin Karakoke, Yasemin Baltacif, Seyithan Taysib. Effect of Nigella sativa on experimental liver fibrosis. Biomedical Research 2014; 25 (1): 32-38.
  33. Yasin TULUCE, Halil OZKOL, Bünyamin SOGUT, Ismail ÇELIK. Effects of igella sativa L. on Lipid Peroxidation and Reduced Glutathione Levels in Erythrocytes of Broiler Chickens. Cell Membranes And Free Radical Research. 2009;1;3-1.
  34. Kanter M, Coskun O, Budancamanak M. Hepatoprotective effects of Nigella sativa L and Urtica dioica L on lipid peroxidation, antioxidant enzyme systems and liver enzymes in carbon tetrachloride- treated rats. World. J. Gastroenterol. 2005;11 (42): 6684-8.
  35. Hanene Jrah Harzallah, Rahma Grayaa, Wafa Kharoubi, Aya Maaloul, Mohamed Hammami, Touhami Mahjoub. Thymoquinone, theNigella sativa Bioactive Compound, Prevents Circulatory Oxidative Stress Caused by 1,2-Dimethylhydrazine in Erythrocyte during Colon Postinitiation Carcinogenesis. Oxidative Medicine and Cellular Longevity. 2012.1-6.
  36. Nabila E Abdelmeguid,Rajaa Fakhoury, Salwa M Kamal, Rana J Al Wafai. Effects of Nigella sativa and thymoquinone on biochemical and subcellular changes in pancreatic β-cells of streptozotocin-induced diabetic rats. J Diabetes 2010 Dec;2(4):256-66.
  37. Tuncel N, Erkasap N, Sahinturk V, Ak DD, Tuncel M. The protective effect of vasoactive intestinal peptide (VIP) on stress-induced gastric ulceration in rats. Ann N Y Acad Sci 1998; 865: 309-322.
  38. Kanter M, Meral I, Dede S, Gunduz H, Cemek M, Ozbek H. Effects of Nigella sativa L. and Urtica dioica L. on lipid peroxidation, antioxidant enzyme systems and some liver enzymes in CCl4- treated rats. J Vet Med A Physiol Pathol Clin Med. 2003;50:264–268.
  39. A. Sayed. Thymoquinone and proanthocyanidin attenuation of diabetic nephropathy in rats. European Review for Medical and Pharmacological Sciences 2012; 16: 808-815.
  40. Bassem Y. Sheikh, Ahmed M. Mohamadin. Thymoquinone a potential therapy for cerebral. Oxidative stress asian journal of natural & Applied Sciences. 2012;1;2.
  41. Dalia A. Hafez. Effects of Nigella sativa Oil and Thymoquinone on Renal Oxidative Stress and Apoptosis Rate in Streptozotocin-Diabetic Rats. Journal of American Science 2013;9(3).
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