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ISSN: 2394-4137 (E) 2394-6318 (P)

IJIMS.2016.109

Type of Article: Original Research

Volume 3; Issue 3: March 2016

Page No.: 248-252

DOI: 10.16965/ijims.2016.109

 

Effect of Nigella Sativa seed and Thymoquinone on Reproductive Parameters in Sterptozotocine Induced Diabetic and Normal Male Albino Rats

Manjunath Aithal 1 , Haseena S *2, Kusal K Das 3, Shaik Hussain Saheb 4.

1 Professor & HOD, Department of  Physiology,  Shri B M Patil Medical College Hospital & Research Centre, Bijapur, Karnataka, India.

*2 Ph.D Scholar, Department of Physiology, Shri B M Patil Medical College Hospital & Research Centre, Bijapur and Lecturer In Physiology, JJM Medical College, Davangere,  Karnataka, India.

3 Professor, Department of  Physiology,  Shri B M Patil Medical College Hospital & Research Centre, Bijapur, Karnataka, India.

4 Assistant professor in Anatomy,  JJM Medical College, Davangere,  Karnataka, India.

CORRESPONDING AUTHOR ADDRESS: Mrs. Haseena S, Lecturer In Physiology, JJM Medical College, Davangere,  Karnataka, India. Mobile No.: +91-9241390409 E-Mail: physiologyshs@gmail.com

ABSTRACT

Introduction: In diabetes an increase in free radicals, DNA damage and lipid peroxidation in human sperm may occur. Antioxidant components in medicinal herbs such as Nigella sativa have been indicated to improve spermatogenesis and steroidogenesis. Thymoquinone and unsaturated fatty acids are the main antioxidant components of Nigella Sativa. Nigella Sativa seeds has been used in traditional Iranian medicine as a natural remedy for promotes females menstruation, galactagogue, carminative, laxative and anti-parasitic properties. The present study is conducted to find out effect of Nigella sativa seed and Thymoquinone on testosterone and LH levels in streptozotocine induced diabetic and normal male albino rats.

Materials and Methods: 36 rats were selected for this study and divided in to 6 groups each contains 6 rats, one group served as normal control, one group served as normal rats received the nigella sativa seed powder(300mg/Kg BW), one groups served as normal rats received the Thymoquinone(4mg/kg BW), one group served as Streptozotocine(50mg/kg BW) induced diabetic control rats,  one group served as diabetic rats  received the nigella sativa seed powder(300mg/Kg BW) and one groups served as diabetic rats received the Thymoquinone(4mg/kg BW).Testosterone and LH were estimated by Chemiluminosis(CLIA).

Results: There is no change in Testosterone and LH levels in normal rats treated with nigella sativa seed powder and thymoqinone. The levels testosterone and LH are decreased significantly in diabetic rats compared with normal rats, when the diabetic groups treated with nigella sativa seed powder and thymoqinone the Testosterone levels are normalised significantly(p<0.05).

Conclusion: Compared with normal rats the level of testosterone was decreased in diabetic rats, when it is treated with Nigella sativa seed and Thymoquinone the levels of testosterone increased significantly. Compared with normal rats the level of LH was decreased significantly in diabetic rats, when it is treated with Nigella sativa seed and `Thymoquinone the levels of LH not significant with other groups.

Key Words: Testosterone, LH, STZ, Diabetes, Thymoquinone, Nigella Sativa seed.

REFERENCES

  1. Salem M L. Immunomodulatory & therapeutic properties of the Nigella sativa L. Intern. Immunopharmacol. 2005;5:1744-770.
  2. Chakravarty H L. Plant wealth of Iraq: A dictionary of plant. Ministry of Agriculture and Agrarian Reform. Baghdad, Iraq. 1976;505.
  3. Riaz M, Syed M and Chaudhary F M. Chemistry of the medicinal plants of genus J Hand Medicus. 1996;39(2):40-45.
  4. Mosher, W.D. and Pratt, W.F. Fecundity and infertility in the United States: incidence and trends. J. Ferti. Steril. 1991;56:192-193.
  5. Jedlinska M, Bomba G, Jakubowski K, Rotkiewicz T, Jana B, Penkowski A. Impact of oxidative stress and supplementation with vitamins E and C on testes morphology in rats. J. Reprod. 2006;52:203-209.
  6. Strzezek R, Koziorowska-Gilun M, Stawiszynska M. Cryopreservation of canine semen: the effect of two extender variants on the quality and antioxidant properties of spermatozoa. Pol J Vet Sci 2012;15(4):721-726.
  7. Aitken RJ, Smith TB, Lord T, Kuczera L, Koppers AJ, Naumovski N, et al. On methods for the detection of reactive oxygen species generation by humanspermatozoa: analysis of the cellular responses to catechol oestrogen, lipid aldehyde, menadione and arachidonic acid. Andrology 2013;1(2):192-205.
  8. Rosenblat M, Volkova N, Aviram M. Pomegranate phytosterol (β-sitosterol) and polyphenolic antioxidant (punicalagin) addition to statin, significantly protected against macrophage foam cells formation. Atherosclerosis 2013;226(1):110-117.
  9. Ateşşahin A, Türk G, Karahan I. Lycopene prevents adriamycininduced testicular toxicity in rats. Fertil Steril 2006;85(Suppl 1):1216-1222.
  10. Stowe CB. The effects of pomegranate juice consumption on blood pressure and cardiovascular health. Complement Ther Clin Pract 2011;17(2):113-115.
  11. Strzezek R, Koziorowska-Gilun M, Stawiszynska M. Cryopreservation of canine semen: the effect of two extender variants on the quality and antioxidant properties of spermatozoa. Pol J Vet Sci 2012;15(4):721-726.
  12. Hala, M. A. Protective effect of Nigella sativa, linseed and celery oils against testicular toxicity induced by sodium valproate in male rats. Journal of American Science 2011;7(5).
  13. Desai S D, Shaik Hussain Saheb, Kusal K Das, Haseena S. Effect of Thymoquinone on MDA and SOD levels in Sterptozotocine Induced Diabetis Albino Rats. Pharm. Sci. & Res. 2015;7(4):206-209.
  14. Desai S D, Shaik Hussain Saheb, Kusal K Das, Haseena S. Effect of Nigella Sativa Seed Powder on MDA and SOD levels in Sterptozotocine Induced Diabetis Albino Rats. Pharm. Sci. & Res. 2015;7(4):523-526.
  15. Haseena S, Manjunath Aithal, Kusal K Das, Shaik Hussain Saheb. Phytochemical Analysis of Nigella Sativa and it`s effect on reproductive system. J. Pharm. Sci. & Res. 2015;7(8):514-517.
  16. Desai S D, Shaik Hussain Saheb, Kusal K Das, Haseena S. Phytochemical Analysis of Nigella Sativa and it`s Antidiabetic Effect. J. Pharm. Sci. & Res. 2015;7(8):527-532.
  17. Murugesan, M. Ragunath, T. Prabu, S. Nadanasabapathi, M. Sakthivel, V. Manju. Protective role of black cumin (Nigella sativa) on isoproterenol induced myocardial infarction in rats. International Journal of Pharmacology and Clinical Sciences. 2012;1(2):45-53.
  18. Rahmatollah Parandin,Namdar Yousofvand,  Rostam Ghorbani. The enhancing effects of alcoholic extract of Nigella sativa seed on fertility potential, plasma gonadotropins and testosterone in male rats. Iran J Reprod Med. 2012 Jul;10(4):355-362.
  19. A. Datau, Wardhana, Eko E. Surachmanto, K. Pandelaki, J.A. Langi, Fias. Efficacy of Nigella Sativa on Serum Free Testosterone and Metabolic Disturbances in Central Obese Male. Acta Med Indones-Indones J Intern Med. 2010;42(3):130-134.
  20. Farooq T, Juma Hayfaa M A Abdulrahman. The effects of Nigella sativa oil administration on some physiological and histological values of reproductive aspects of rats. Iraqi J. Vet. Med. 2011;35(2):52- 60.
  21. Houghton PJ, Zarka R, De las Heras B, Hoult JR. Fixed oil of Nigella sativa and derived Thymoquinone inhibit eicosanoid generation in leukocytes and membrane lipid peroxidation. Planta Med 1995;61:33-36.
  22. Turkdogan MK, Agaoglu Z, Yener Z, Sekeroglu R, Akkan HA, Avci ME. The role of antioxidant vitamins (C and E), selenium and Nigella sativa in the prevention of liver fi brosis and cirrhosis in rabbits: new hopes. Dtsch Tierarztl Wochenschr 2001;108:71-73.
  23. Akhondian J, Parsa A, Rakhshande H. The effect of Nigella sativa (black cumin seed) on intractable pediatric seizures. Med Sci Monit 2007;13:555-559.
  24. WHO: protocol CG-04. Preparation of alcoholic extract for bioassay and phytochemical studies (APJF/IP, 1001 A). Geneva, World Health Organization;1983.
  25. Singh J, O’Neill C, Handelsman DJ. Induction of spermatogenesis by androgens in gonadotropin-deficient (hpg) mice. Endocrinology 1995;136:5311-5321.
  26. Bhasin S, Fielder T, Peacock N, Sod-Mariah UA, Swerdloff RS. Dissociating antifertility effects of GnRH antagonist from its adverse effects on mating behavior in male rats. Am J Physiol Endocrinol Metab 1988;254:84-91.
  27. O’Donnel L, Mc LachlanRI, Wreford NG, Robertson DM. Testosterone promotes the conversion of round spermatids between stages vii and viii of the rat spermatogenic cycle. Endocrinology 1994;135:2608-614.
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