Type of Article: Original Research

Volume 5; Issue 9: 2018

Page No.: 736-740

DOI: 10.16965/ijims.2018.131


K. Kishore Kumar ¹, K.Sai Samrat *2, S. Sony Reddy 2.

1 Assistant Professor, Department Of Pulmonary Medicine,  Chalmeda Anand Rao Institute Of Medical Sciences,Bommakal, Karimnagar, Telangana, India.

*2 Junior Resident,  Department Of Pulmonary Medicine,  Chalmeda Anand Rao Institute Of Medical Sciences,Bommakal, Karimnagar, Telangana, India.

Address for Correspondence:  Dr. K. Sai Samrat, Junior Resident,  Department Of Pulmonary Medicine,  Chalmeda Anand Rao Institute Of Medical Sciences,Bommakal, Karimnagar, Telangana, India. E-Mail: saisamratk.78@gmail.com


Aims/Objectives To compare the efficacy of light emitting diode fluorescent microscopy (LED) over conventional sputum smear microscopy in diagnosing  Mycobacterium Tuberculosis.

Materials and Methods: This comparative study was conducted in the department of Pulmonology In  Chalmeda Anand Rao Institute Of Medical Sciences, Karimnagar from November-2015 to June- 2017. A total of 100 sputum samples were collected from patients suspected of pulmonary TB.

Results: Out of 100 cases which were included in the study, 17 cases showed sputum positivity on AO staining where as only 11 cases showed sputum positivity on ZN staining. Number of  paucibacillary cases detected on AO staining were 7, compared to only 2 on ZN staining.

Conclusion:  Hence our study concludes that AO staining with LED is more efficient over ZN stain in detecting Tuberculosis bacilli in sputum, especially the paucibacillary cases in the diagnosis TB.

Key Words: Mycobacterium Tuberculosis, Sputum examination, Ziehl Neelsen Staining(ZN staining), Auramine-O Staining (AO staining).


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